HOME
DNA restriction analysis
DNA can be isolated in the Genetics laboratory from a variety of animal tissues (e.g. liver, muscle, blood) using different techniques, depending on the kind of tissue and the method of preservation. Fish samples from the Bay of Biscay and the Gulf of Guinea have been successfully transported to Crete and used for DNA isolation. The Department has applied restriction analysis, especially of mitochondrial DNA (mtDNA), which is a very powerful tool for studying genetic polymorphism, in several fish species: sardine, anchovy, swordfish, gilthead sea bream
Polymerase Chain Reaction (PCR) amplification.
The most modern technique, allowing a short DNA segment to be amplified enormously using specific primers (oligonucleotides) PCR amplification has been successfully applied for different regions of fish mtDNA (D-loop, Cyto b, etc) as well as in microsatellite DNA
The sequence of nucleotides on the chain of DNA can be determined according to the Dideoxy method, using Poly-Acrylanide gel electrophoresis.
The whole range of microsatellite DNA analysis has successfully been carried out for the gilthead sea bream and already one of the broodstock of IMBC has been genetically tagged. The application of this method has already been initiated in a number of other marine species (Sparidae species, swordfish, catfish, anchovy, oyster).
The Department has extensive experience in scoring allozyme polymorphism in a variety of marine species, such as sardine, anchovy, and oysters.
Allozymes can provide useful insights into the population structure of natural stocks, the degree of inbreeding of both natural and reared populations, the presence of hybrids, etc.
WHAT'S NEW - THE INSTITUTE - PROJECTS AND PROGRAMMES - CASE STUDIES
BIBLIOGRAPHICAL SERVICES - PRODUCTS & SERVICES - INTERNET RESOURCES