Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop (Placopecten magellanicus) and the Iceland Scallop (Chlamys islandica) <B>Use of Biochemical Genetic Markers to Discriminate between Adductor Muscles of the Sea Scallop (Placopecten magellanicus) and the Iceland Scallop (Chlamys islandica)</B><P> E. Kenchington</B><I><P> Department of Fisheries and Oceans, Biological Sciences Branch, Scotia-Fundy Region, P.O. Box 550, Halifax, NS B3J 2S7, Canada</I><B><P> K.S. Naidu</B><I><P> Department of Fisheries and Oceans, Science Branch, P.O. Box 5667, St. John's </I>NF A1C 5X1, <I>Canada</I><B><P> D.L. Roddick</B><I><P> Department of Fisheries and Oceans, Biological Sciences Branch, Scotia-Fundy Region, P.O. Box 550, Halifax, NS B3J 2S7, Canada</I><B><P> D.I. Cook and E. Zouros</B><I><P> Biology Department, Dalhousie University, Halifax, NS B3H 4J1, Canada</I><B><P> Kenchington, E., K.S. Naidu, D. L. Roddick, D.I. Cook, and E. Zouros. 1993. Use of biochemical genetic markers to discriminate between adductor muscles of the sea scallop </B><I><B>(Placopecten magellanicus) </B></I><B>and the Iceland scallop </B><I><B>(Chlamys islandica). </B></I><I> Can. J. Fish. Aquat. Sci.</I> 50: 1222-1228.<P> Three biochemical techniques were applied to adductor muscles of <I>Placopecten magellanicus </I>and <I> Chlamys islandica, </I>two commercially important scallops, to search for species-specific genetic markers. Allozyme electrophoresis identified two enzyme systems, mannose phosphate isomerase and glutamic oxaloacetate transaminase, which appear to be diagnostic. Sequencing of the 18S rRNA gene in each species showed 15 nucleotide differences over 1815 base pairs. Digestion of the gene with the restriction enzyme XHO I released two fragments in <I>Placopecten </I> and three in <I>Chlamys. </I>All three techniques could be developed for management purposes as part of an enforcement programme to identify illegally caught scallops.<P> Nous avons applique trois techniques biochimiques aux muscles adducteurs de <I>Placopecten magellanicus </I>et <I>Chlamys islandica, </I>deux especes de petoncles commercialement importantes, pour rechercher des marqueurs genetiques particuliers A chaque espece. L'electrophorese des allozymes a permis d'identifier deux systemes enzymatiques, la mannose-phosphate-isomerase et la glutamate-oxaloacetate-transaminase, qui semblent avoir un pouvoir diagnostique. Le sequencage du gene 18S de l'ARNr chez chaque espece a revele 15 differences dans les nucleotides sur 1 815 paires de bases. La digestion du gene par l'enzyme de restriction XHO I a libere deux fragments chez <I>Placopecten </I>et trois chez <I>Chlamys. </I>Il serait possible de developper ces trois techniques a des fins de gestion dans le cadre d'un programme d'application des reglements visant l'identification des petoncles captures illegalement.<P> Received September 10, 1992Recu le 10 septembre 1992<BR> Accepted December 9, 1992 Accepte le 9 decembre 1992 <I><P> Can. J Fish. Aquat. Sci., </I>Vol. 50, 1993, 1222-1227<CENTER></CENTER><P>