Native carbon mineralization of an acid organic soil after use of the chloroform-fumigation method to estimate microbial biomass
Native carbon mineralization of an acid organic soil after use of the chloroform-fumigation method to estimate microbial biomassM.M. Couteaux(l), R. Henkinet(l), P. Pitta(l), P. Bottner(2),
G. Billes(2), L. Palka(l), and G. Vannier(l)
(l )CNRS/UA 689, Museum National d'Histoire Naturelle,
Laboratoire d'Ecologie Generale, 4, avenue du Petit
Chateau, F-91800 Brunoy, France
(2) CNRS/CEPE, B.P. 5051, F-34033 Montpellier Cedex,
France
Summary. Two experiments were carried out on a dysmoder
humus sampled from a depth of 2- 5 cm from a mixed
hardwood forest. In acid soil, the chloroform fumigation-incubation
method failed to estimate the microbial biomass, not
because bacterial growth was inhibited after fumigation
but because a labile C source was taken up which differed
from the killed biomass C.
Key words: Biomass - Chloroform fumigation-incubation
technique - Acid organic soil - Carbon mineralization
Biol Fertil Soils (1989) 8:172- 177